Humanized Anti-NCAM1 Recombinant Antibody (clone Lorvotuzumab) (CAT#: TAB-188)
This product is a humanized (from mouse) IgG1, κ antibody that can recognize human NCAM1.
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Custom Production
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DB
Figure 1 Anti-Human NCAM1 Therapeutic Antibody (TAB-188) in DB
Dot Blot analysis of TAB-188 was performed by coating with human NCAM1 protein (His tag).
TAB-188 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 6000)
WB
Figure 2 Anti-Human NCAM1 Therapeutic Antibody (TAB-188) in WB
Western blot analysis of TAB-188 was performed with human NCAM1 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-188 and HRP goat anti-Human IgG was used as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 3 Anti-Human NCAM1 Therapeutic Antibody (TAB-188) in ELISA
ELISA analysis of TAB-188 was performed by coating with human NCAM1 protein (His tag). Then blocked with BSA and incubated with TAB-188. The HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- Humanized (from mouse) IgG1, κ
- Specificity
- Human NCAM1
- Species Reactivity
- Human
- Clone
- Lorvotuzumab
- Applications
- ELISA, FC, IP, FuncS, IF, Neut, ICC
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization, Immunocytochemistry.
Target
- Alternative Names
- CD56; NCAM; MSK39; neural cell adhesion molecule 1; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule, NCAM
- Gene ID
- 4684
- UniProt ID
- P13591
Related Resources
Please refer to Lorvotuzumab Mertansine Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lorvotuzumab Mertansine.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-188, RRID: AB_3111857)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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